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dc.contributor.authorÖz, Umut Can
dc.contributor.authorBolat, Zeynep Büşra
dc.contributor.authorÖzköse, Umut Uğur
dc.contributor.authorGülyüz, Sevgi
dc.contributor.authorKüçüktürkmen, Berrin
dc.contributor.authorKhalily, Melek Parlak
dc.date.accessioned2021-06-05T19:56:23Z
dc.date.available2021-06-05T19:56:23Z
dc.date.issued2021
dc.identifier.issn0928-4931
dc.identifier.issn1873-0191
dc.identifier.urihttps://doi.org/10.1016/j.msec.2021.111929
dc.identifier.urihttps://hdl.handle.net/20.500.12960/255
dc.description0000-0002-5402-0467en_US
dc.description0000-0002-9216-6336en_US
dc.description0000-0003-3892-2775en_US
dc.descriptionPubMed: 33812571en_US
dc.descriptionWOS:000636847800005en_US
dc.description.abstractThe equipping of nanoparticles with the peptide moiety recognizing a particular receptor, enables cell or tissue specific targeting, therefore the optimization of the targeted nanoparticles is a key factor in the formulation design process. In this paper, we report the optimization concept of Doxorubicin encapsulating PEtOx-b-PLA polymersome formulation equipped with Peptide18, which is a breast cancer recognizing tumor homing peptide, and the unveiling of the cell-specific delivery potential. The most dominant formulation parameters, which are the polymer to Doxorubicin mass ratio (w/w) and the aqueous to organic phase ratio (v/v), were optimized using Central Composite Design (CCD) based Response Surface Methodology. The characteristics of optimum polymersome formulation were determined as the hydrodynamic diameter of 146.35 nm, the PDI value of 0.136, and the encapsulation efficiency of 57.11% and TEM imaging, which are in agreement with the DLS data, showed the spherical morphology of the polymersomes. In order to demonstrate the breast cancer-specific delivery of targeted polymersomes, the flow cytometry and confocal microscopy analyses were carried out. The targeted polymersomes were accumulated 8 times higher in AU565 cells compared to MCF10A cells and the intracellular Doxorubicin was almost 10 times higher in AU565 cells. The CCD-mediated optimized targeted polymersomes proposed in this report holds the promise of targeted therapy for breast cancer and can be potentially used for the development of novel treatments.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [213M760]en_US
dc.description.sponsorshipAcknowledgements This research was financially supported by The Scientific and Technological Research Council of Turkey (TUBITAK, 213M760) .en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.ispartofMaterials Science & Engineering C-Materials for Biological Applicationsen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCentral Composite Designen_US
dc.subjectDoxorubicinen_US
dc.subjectPeptide18en_US
dc.subjectPetox-Plaen_US
dc.subjectPolymersomeen_US
dc.subjectTargeted Deliveryen_US
dc.titleA robust optimization approach for the breast cancer targeted design of PEtOx-b-PLA polymersomesen_US
dc.typearticleen_US
dc.departmentFen Edebiyat Fakültesi, Kimya Bölümüen_US
dc.department-temp[Oz, Umut can; Kucukturkmen, Berrin; Bozkir, Asuman] Ankara Univ, Fac Pharm, Dept Pharmaceut Technol, TR-06560 Ankara, Turkey; [Bolat, Zeynep Busra; Telci, Dilek; Sahin, Fikrettin] Yeditepe Univ, Fac Engn & Architecture, Dept Genet & Bioengn, 26 Agustos campus, TR-34755 Istanbul, Turkey; [Bolat, Zeynep Busra] Istanbul Sabahattin Zaim Univ, Fac Engn & Nat Sci, Dept Mol Biol & Genet, TR-34303 Istanbul, Turkey; [Ozkose, Umut Ugur; Gulyuz, Sevgi; Yilmaz, Ozgur] TUBITAK, Marmara Res ctr, Mat Inst, TR-41470 Kocaeli, Turkey; [Ozkose, Umut Ugur; Gulyuz, Sevgi] Istanbul Tech Univ, Fac Sci & Letters, Dept chem, TR-34469 Istanbul, Turkey; [Ozkose, Umut Ugur] Piri Reis Univ, Fac Sci & Letters, Dept chem, TR-34940 Istanbul, Turkey; [Khalily, Melek Parlak] Yozgat Bozok Univ, Fac Sci & Letters, Dept chem, TR-66200 Yozgat, Turkey; [Ozcubukcu, Salih] Middle East Tech Univ, Dept chem, Fac Sci, TR-06800 Ankara, Turkey; [Esendagli, Gunes] Hacettepe Univ, Inst canc, Dept Basic Oncol, TR-06100 Ankara, Turkeyen_US
dc.contributor.institutionauthorÖzköse, Umut Uğur
dc.identifier.doi10.1016/j.msec.2021.111929
dc.identifier.volume123en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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